Transformation Protocol

Transformations are essential to using the distribution kits sent out by the Registry. They can also be one of the more fickle laboratory techniques. We recommend the following protocol as it is the same one used at iGEM HQ. We have tested transformations of the distribution kit with this protocol and have found that it is the best protocol to use with Registry parts and ensures the highest efficiency for the transformation. This protocol may be particularly useful if you are finding that your transformations are not working, or yielding few colonies. At iGEM HQ we make our own stocks of NEB 10beta competent cells. Competent cells purchased from vendors will have better efficiency.